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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in lots of tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential function in promoting mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been explained. Two added splice variants had been claimed but detailed nucleotide sequence details is not nevertheless accessible.
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Blocking peptides are peptides that bind specially into the goal antibody and block antibody binding. These peptide commonly contains the epitope acknowledged via the antibody.
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This manual outlines further attainable optimization of cellular and immunoassay parameters to make sure the very best effects are...
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This guideline outlines further probable optimization of cellular and immunoassay parameters to make certain the best possible results are...
Antibodies bound to the blocking peptide no more bind towards the epitope to the concentrate on protein. By evaluating the staining through the blocked antibody compared to the antibody on your own, you can see which staining is specific.
Concentrate on Info Cyclin E belongs towards the highly conserved cyclin loved ones, whose customers are characterised by a extraordinary periodicity in protein abundance through the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of every mitotic occasion. Cyclin E sorts a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary check here and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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